The process of mutation analysis involves several steps:
1. Sample Collection: Tissue samples are collected from the patient, often through a biopsy. 2. DNA Extraction: DNA is extracted from the collected tissue samples. 3. Amplification: The extracted DNA is amplified using techniques like Polymerase Chain Reaction (PCR) to make enough copies for analysis. 4. Sequencing: The amplified DNA is sequenced to identify any mutations. Techniques such as Sanger sequencing or next-generation sequencing (NGS) are commonly used. 5. Data Analysis: The sequenced data is analyzed using bioinformatics tools to identify and interpret the mutations.
