Several challenges can affect the accuracy and reproducibility of MVD measurements:
1. Tissue Heterogeneity: Different regions of a tissue sample may have varying levels of vascularization, making it difficult to obtain consistent measurements. 2. Staining Variability: The quality and intensity of staining can vary between samples, potentially leading to under- or overestimation of MVD. 3. Manual Counting: Manual counting of microvessels is time-consuming and prone to observer bias. Automated methods can mitigate some of these issues but require careful calibration.
