Several histological and molecular biology techniques are employed to study CYP7A1. Immunohistochemistry (IHC) is commonly used to localize and visualize CYP7A1 in liver tissues. Western blotting and RT-PCR (reverse transcription-polymerase chain reaction) are employed to measure its protein and mRNA expression levels, respectively. These techniques help in understanding the enzyme's regulation, function, and role in various pathologies.
