What are the Alternatives to Cytochrome c Oxidase Staining?
Several alternative methods exist for assessing mitochondrial function, including:
Spectrophotometric Assays: These assays measure the activity of COX and other mitochondrial enzymes in tissue homogenates or isolated mitochondria. Immunohistochemistry: Using antibodies against COX subunits to visualize enzyme levels in tissue sections. Western Blotting: Quantifying COX protein levels in tissue or cell lysates. Mitochondrial DNA Analysis: Detecting mutations or deletions in mitochondrial DNA that affect COX function.
