What are the Limitations of Cytochrome c Oxidase Staining?
Despite its usefulness, cytochrome c oxidase staining has several limitations:
Sensitivity: The staining method may not detect low levels of COX activity, leading to false negatives. Specificity: Non-specific staining can occur, complicating the interpretation of results. Technical Variability: The staining process can be affected by tissue preparation, incubation times, and reagent quality, leading to variability in results. Quantification: The method is primarily qualitative, making it challenging to quantify COX activity accurately.
