Ongoing research and technological advancements are continually improving CISH. New probe designs and labeling techniques are enhancing the specificity and sensitivity of the method. Additionally, advances in imaging and digital pathology are improving the analysis and quantification of CISH signals. Combining CISH with other molecular techniques, such as immunohistochemistry (IHC), is also expanding its applications. These combined approaches allow for the simultaneous detection of nucleic acids and proteins, providing a more comprehensive understanding of tissue biology and disease mechanisms.