What are the Steps Involved in Paraffin Embedding?
The process involves several key steps:
Fixation: The tissue is preserved using fixatives like formalin to prevent degradation. Dehydration: The tissue is gradually dehydrated using increasing concentrations of alcohol to remove water. Clearing: A clearing agent, such as xylene, replaces the alcohol, making the tissue transparent. Infiltration: The tissue is infiltrated with molten paraffin wax. Embedding: The tissue is embedded in a block of paraffin wax, which is then cooled to harden.
