Routine stains are applied through a series of steps involving fixation, embedding, sectioning, and staining of the tissue sample.
Fixation: Preserves tissue morphology and prevents degradation. Embedding: Infiltrates the tissue with a medium (e.g., paraffin) to provide structural support. Sectioning: Thin slices of the embedded tissue are cut using a microtome. Staining: The sections are treated with the appropriate stain to highlight specific structures.
