There are several staining techniques used to visualize myelin in histological sections:
Luxol Fast Blue (LFB): A commonly used dye that binds to the lipoproteins in myelin, providing a blue coloration to myelinated fibers while leaving the background and other tissue components relatively unstained. Weil's Method: This technique involves the use of hematoxylin and differentiating agents to selectively stain myelin, resulting in a black coloration of myelinated fibers. Osmium Tetroxide: A heavy metal stain that binds to lipids, producing a black coloration of myelin. It is often used for electron microscopy due to its high contrast and preservation of ultrastructure.
