The Luxol Fast Blue staining procedure involves several steps:
Fixation: Tissue samples are fixed using a chemical fixative, such as formalin, to preserve the tissue structure. Embedding: The fixed tissue is embedded in a paraffin block to facilitate sectioning. Sectioning: Thin sections of the embedded tissue are cut using a microtome. Staining: The tissue sections are treated with Luxol Fast Blue solution and incubated, allowing the dye to bind to the myelin. Differentiation: The sections are differentiated using a lithium carbonate solution to remove excess dye, leaving only the myelinated fibers stained blue. Counterstaining: Hematoxylin or other counterstains may be used to enhance the contrast of cellular structures.
