Biochemists use a variety of techniques to study tissues at the molecular level. Some of the most important techniques include:
Mass Spectrometry: This technique helps identify and quantify proteins, lipids, and other molecules in tissue samples. Western Blotting: Used to detect specific proteins within a tissue sample, providing insights into protein expression and modifications. PCR (Polymerase Chain Reaction): Amplifies DNA sequences to study gene expression and genetic variations in tissues. Fluorescence Microscopy: Allows visualization of specific molecules within tissues using fluorescent tags. Chromatography: Used to separate and analyze complex mixtures of biomolecules in tissue samples.
