How is Precipitating Used in Immunohistochemistry?
In immunohistochemistry (IHC), precipitating is often employed in the detection of specific antigens. Enzyme-conjugated antibodies react with a substrate to produce a colored precipitate at the site of antigen-antibody binding. For instance, the enzyme horseradish peroxidase (HRP) reacts with diaminobenzidine (DAB) to produce a brown precipitate, indicating the presence of the target antigen.
