Selecting an appropriate endogenous control is critical for accurate data normalization. The selected control gene or protein should have stable and consistent expression levels under the specific experimental conditions. Here are some criteria to consider:
1. Consistency Across Samples: The expression of the control should not vary significantly between different samples or experimental groups. 2. Expression Levels: The control should be expressed at a similar level to the target gene or protein to avoid issues with normalization. 3. Validation: It is essential to validate the stability of the control's expression in the specific experimental setup being used.
