What Techniques are Used to Study Tregs in Histology?
Several advanced techniques are employed to study Tregs in histological contexts. Immunohistochemistry (IHC) is one of the primary methods, allowing for the visualization of Tregs in tissue sections using specific antibodies. Flow cytometry is another powerful technique that can quantify and characterize Tregs based on their surface markers and intracellular proteins. Additionally, in situ hybridization and RNA sequencing can provide insights into the gene expression profiles of Tregs under various conditions.