While the Nissl stain is highly effective for certain applications, it does have some limitations:
Limited Specificity: It primarily stains the rough endoplasmic reticulum and does not provide much information about other cellular components. Lack of Subcellular Detail: The stain does not offer detailed visualization of subcellular structures such as synapses or dendrites. Tissue Preparation: The staining process can be affected by the quality of tissue fixation and sectioning.
