Preventing chattering involves addressing the factors that cause it. Here are some tips:
- Adjust blade angle: Ensure the microtome blade is set to the correct angle for the type of tissue being sectioned. - Optimize fixation: Use an appropriate fixation protocol to avoid over-hardening of the tissue. - Proper embedding: Ensure that tissues are embedded smoothly and evenly in paraffin wax. - Maintain equipment: Regularly check and maintain the microtome to ensure all parts are functioning correctly.
