Chattering can be caused by several factors, including:
- Incorrect blade angle: The microtome blade angle needs to be set correctly. A blade angle that is too steep or too shallow can result in chattering. - Tissue hardness: Variations in tissue hardness, for example, due to excessive fixation or calcification, can lead to chattering. - Improper embedment: Inconsistent or improper embedding of the tissue in paraffin wax can create uneven resistance during sectioning, leading to chattering. - Microtome issues: Mechanical problems with the microtome, such as a loose blade holder or worn-out components, can also cause chattering.
