What is Chattering?
Chattering refers to a series of fine, repetitive, and regular vertical lines seen in histological sections. These lines are often artifacts produced during the sectioning of tissue samples with a microtome. The term "chattering" is derived from the visual resemblance to the repeated, regular patterns that resemble the noise of chattering.
- Incorrect blade angle: The microtome blade angle needs to be set correctly. A blade angle that is too steep or too shallow can result in chattering.
- Tissue hardness: Variations in tissue hardness, for example, due to excessive fixation or calcification, can lead to chattering.
- Improper embedment: Inconsistent or improper embedding of the tissue in paraffin wax can create uneven resistance during sectioning, leading to chattering.
- Microtome issues: Mechanical problems with the microtome, such as a loose blade holder or worn-out components, can also cause chattering.
- Adjust blade angle: Ensure the microtome blade is set to the correct angle for the type of tissue being sectioned.
- Optimize fixation: Use an appropriate fixation protocol to avoid over-hardening of the tissue.
- Proper embedding: Ensure that tissues are embedded smoothly and evenly in paraffin wax.
- Maintain equipment: Regularly check and maintain the microtome to ensure all parts are functioning correctly.
Why is it Important to Avoid Chattering?
Avoiding chattering is crucial because it can obscure
histological details, making it difficult to accurately interpret tissue morphology. This can lead to misdiagnosis or inaccurate research conclusions, particularly in pathology.
How is Chattering Detected?
Chattering is typically detected during the microscopic examination of histological sections. The characteristic repetitive vertical lines are a clear indication. Laboratory personnel should be vigilant and inspect sections for artifacts before proceeding with further staining or analysis.
Can Chattering be Corrected?
Once chattering has occurred, it is usually not possible to correct it in the affected sections. However, steps can be taken to re-section the tissue if enough material is available. Adjustments to the microtome settings or re-embedding the tissue may be necessary to obtain high-quality sections.
What are the Consequences of Ignoring Chattering?
Ignoring chattering can have significant consequences. In diagnostic settings, it can lead to inaccurate diagnoses, which can affect patient care. In research, it can compromise the validity of the results, leading to potential setbacks in scientific understanding or misinformed conclusions.
Conclusion
Chattering is a common artifact in histology that can significantly impact the quality of histological sections. By understanding its causes, detection methods, and prevention strategies, laboratory personnel can minimize its occurrence and ensure the accuracy and reliability of histological analyses.
