To achieve the best results in cryo sectioning, consider the following tips:
1. Proper Freezing: Ensure rapid and uniform freezing of the tissue to minimize ice crystal formation. 2. Optimal Temperature: Maintain the cryostat at the ideal temperature for the specific tissue type being sectioned. 3. Sharp Blades: Use sharp, high-quality blades to produce clean cuts and reduce artifacts. 4. Correct Embedding Medium: Use an appropriate embedding medium like OCT compound to provide support and protection to the tissue during sectioning.
