Cryo sectioning is employed in various applications, including:
1. Histochemical Staining: The technique is frequently used for histochemical staining, which requires the preservation of enzyme activity. 2. Immunohistochemistry: Cryo sections are ideal for immunohistochemical studies because they preserve antigenicity better than paraffin-embedded sections. 3. Fluorescence Microscopy: The method is also used in fluorescence microscopy to study cellular structures and protein localization.