To achieve quality sectioning, consider the following tips:
Proper Fixation: Ensure thorough fixation to maintain tissue integrity. Optimal Embedding: Use the appropriate embedding medium and technique for the tissue type. Regular Maintenance: Keep the microtome and blades in good condition through regular maintenance and cleaning. Training: Adequate training and experience are essential for technicians to produce high-quality sections. Environmental Control: Maintain suitable environmental conditions, such as temperature and humidity, to prevent artifacts.