Advantages: - Speed: Cryo sectioning is much faster than traditional paraffin embedding and sectioning. - Preservation of Native State: Better preserves the native state of the tissue, including enzymes and antigens. - No Need for Dehydration and Clearing: Eliminates steps that can cause artifacts in tissue morphology.
Disadvantages: - Tissue Morphology: The freezing process can sometimes cause ice crystal artifacts, which may distort tissue morphology. - Temperature Sensitivity: Requires precise temperature control to ensure optimal sectioning quality. - Limited Long-term Storage: Frozen sections are generally less stable for long-term storage compared to paraffin-embedded sections.
