The process of cryo sectioning involves several key steps:
1. Tissue Freezing: The tissue sample is rapidly frozen using a cryostat, which can reach temperatures as low as -20°C to -30°C. The freezing medium, often OCT (Optimal Cutting Temperature) compound, is used to embed and support the tissue. 2. Sectioning: Once the tissue is frozen, it is cut into very thin sections (usually between 5-20 micrometers) using a microtome within the cryostat. 3. Mounting: The sections are then mounted onto glass slides for further analysis. The sections can be stained immediately or stored for later use.
