In Histology, the study of PI3K involves various techniques such as immunohistochemistry (IHC), western blotting, and fluorescence microscopy. IHC can be used to detect the expression and localization of PI3K and its downstream targets in tissue sections. Western blotting allows for the quantification of PI3K protein levels and its phosphorylation status. Fluorescence microscopy, often coupled with fluorescently labeled antibodies, enables the visualization of PI3K signaling pathways in live cells.
