The fixation process generally involves the following steps:
1. Specimen Collection: Fresh tissue samples are collected quickly to prevent degradation. 2. Fixative Application: The tissue is immersed in the fixative solution, ensuring complete penetration. 3. Incubation: The tissue is allowed to fix for a specified period, depending on the fixative and tissue type. 4. Rinsing: The fixed tissue is rinsed to remove excess fixative, which may interfere with subsequent processing.
