NeuN is typically detected using immunohistochemistry (IHC) or immunofluorescence (IF) techniques. These techniques involve using antibodies that specifically bind to the NeuN protein, allowing for its visualization under a microscope. The process generally includes:
Fixation of tissue samples to preserve cellular structures. Incubation with a primary antibody specific to NeuN. Application of a secondary antibody conjugated to a detectable marker (e.g., an enzyme or fluorophore). Visualization using appropriate detection methods.
