Gene synthesis involves several steps: 1. Design: The desired DNA sequence is designed using specialized software. 2. Synthesis: Short DNA fragments, known as oligonucleotides, are chemically synthesized. 3. Assembly: These oligonucleotides are assembled into the full-length gene using techniques such as polymerase chain reaction (PCR) or ligation. 4. Verification: The synthesized gene is sequenced to ensure it matches the desired sequence.
