What are the Different Techniques in High Resolution Microscopy?
Several techniques fall under the umbrella of high resolution microscopy:
Confocal Microscopy: Uses laser light to scan the sample and create optical sections, providing high-resolution, three-dimensional images. Fluorescence Microscopy: Utilizes fluorescent dyes to label specific components of the cells, enhancing the visualization of cellular structures. Electron Microscopy: Includes Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM), both of which use electrons instead of light to achieve much higher resolutions. Super-Resolution Microscopy: Techniques like STED, PALM, and STORM surpass the diffraction limit of light, allowing for visualization at the nanometer scale.