CHOP is an essential step in the process of tissue processing and embedding. Once a tissue sample has been fixed and embedded in a medium like paraffin wax, it is secured onto a microtome. The microtome then uses a steel, glass, or diamond blade to make thin slices of the tissue, usually between 3 to 10 micrometers thick. These thin sections are then mounted on a slide and stained to highlight various cellular components.
