What are the optimal conditions for tissue fixation?
Tissue fixation is a critical step in histology to preserve tissue structure and prevent degradation. The optimal conditions for fixation include using a suitable fixative like formaldehyde or glutaraldehyde at the correct concentration, typically around 10% for formaldehyde. The tissue should be adequately immersed and the fixation time should be optimized, usually ranging from 6 to 48 hours depending on the tissue type and size. Temperature control is also essential; fixation is typically performed at room temperature (20-25°C) to balance fixation speed and preservation quality.
