What role does section thickness play in histological analysis?
Section thickness is critical in histological analysis, as it influences the clarity and detail of observed structures. Typical paraffin sections are cut at 4-5 micrometers, which balance the need for detail and the structural integrity of the tissue. Thicker sections may obscure fine details, while thinner sections can be more challenging to handle and may tear. Consistent section thickness is essential for reproducible results, particularly in comparative studies or quantitative analyses.
