How does temperature affect histological processes?
Temperature plays a crucial role in various histological processes, including fixation, embedding, and staining. During fixation, higher temperatures can accelerate chemical reactions, potentially improving fixation speed but risking over-fixation and morphological artifacts. During embedding in paraffin, maintaining the paraffin at 56-58°C ensures proper infiltration without overheating the tissue. Staining procedures also benefit from controlled temperatures, as some dyes require specific conditions to bind effectively. Consistent temperature regulation ensures reproducibility and quality of histological preparations.
