The staining process involves several steps. Initially, the tissue sample is treated with a silver nitrate solution. Silver ions are then reduced to metallic silver by a reducing agent, usually formalin or hydroquinone. This reaction selectively stains nerve fibers and other components, making them appear dark under the microscope. The stain highlights the intricate details of neural structures, which are otherwise difficult to see with conventional stains like Hematoxylin and Eosin.
