Once the sample is collected through aspiration, it undergoes several steps to prepare it for histological examination:
1. Smearing: The aspirate is smeared onto glass slides. 2. Fixation: The slides are fixed using chemicals like alcohol to preserve cellular details. 3. Staining: Various stains, such as Giemsa or Papanicolaou, are applied to enhance cellular structures. 4. Microscopic Examination: The stained slides are examined under a microscope by a pathologist.