What is Two Dimensional Gel Electrophoresis (2-DE)?
Two dimensional gel electrophoresis (2-DE) is a powerful technique used to separate complex mixtures of proteins. It combines two different electrophoretic separation methods: isoelectric focusing (IEF) and SDS-PAGE. First, proteins are separated based on their isoelectric point (pI) during IEF, then based on their molecular weight during SDS-PAGE. This results in a high-resolution map of proteins that can be analyzed for various applications.
