The process of secondary antibody incubation involves several steps: 1. Blocking: Before incubation, sections are blocked with a blocking buffer to prevent non-specific binding. 2. Incubation: The tissue sections or cells are incubated with the secondary antibody, usually for 30 minutes to 1 hour at room temperature or overnight at 4°C. 3. Washing: After incubation, sections are washed several times with a buffer like PBS or TBS to remove unbound antibodies, reducing background noise.
