Incomplete fixation can be identified through various signs, including:
1. Poor Morphology: Tissue sections may appear distorted or shrunken. 2. Inconsistent Staining: Uneven staining patterns, especially in deeper tissue regions. 3. Cellular Artifacts: Presence of artifacts such as cell shrinkage, vacuolation, or nuclear pyknosis. 4. Autolysis Signs: Evidence of cellular degradation, such as loss of nuclear detail and cytoplasmic integrity.
