incomplete fixation

How can incomplete fixation be avoided?

Preventing incomplete fixation involves several best practices:
1. Adequate Fixative Volume: Ensure a fixative-to-tissue ratio of at least 10:1 to facilitate proper penetration.
2. Optimal Tissue Size: Trim tissue samples to an appropriate size, typically no thicker than 5 mm.
3. Sufficient Fixation Time: Allow adequate time for fixation, typically 24-48 hours depending on tissue type and thickness.
4. Use of Appropriate Fixative: Select a fixative suitable for the specific tissue and intended analysis.
5. Maintain Fixative Quality: Use fresh, uncontaminated fixative solutions and store them properly.

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