Preventing incomplete fixation involves several best practices:
1. Adequate Fixative Volume: Ensure a fixative-to-tissue ratio of at least 10:1 to facilitate proper penetration. 2. Optimal Tissue Size: Trim tissue samples to an appropriate size, typically no thicker than 5 mm. 3. Sufficient Fixation Time: Allow adequate time for fixation, typically 24-48 hours depending on tissue type and thickness. 4. Use of Appropriate Fixative: Select a fixative suitable for the specific tissue and intended analysis. 5. Maintain Fixative Quality: Use fresh, uncontaminated fixative solutions and store them properly.