What should be done if incomplete fixation is suspected?
If incomplete fixation is suspected, several steps can be taken:
1. Re-fixation: Re-fix the tissue using an adequate volume and appropriate fixative. 2. Sectioning and Reprocessing: Re-sectioning the tissue into smaller pieces before re-fixation can enhance fixative penetration. 3. Quality Control: Implement rigorous quality control measures to monitor fixation effectiveness and address issues promptly.
In conclusion, achieving complete fixation is paramount for obtaining reliable and high-quality histological results. By understanding the causes, consequences, and preventive measures, histologists can ensure optimal tissue preservation and accurate analysis.
