What Techniques are Used to Study Cell Signaling Pathways in Histology?
Several advanced techniques are employed to study cell signaling pathways: - Immunohistochemistry (IHC): Allows visualization of specific signaling molecules within tissue sections using antibodies. - In situ Hybridization (ISH): Detects specific nucleic acid sequences within tissue sections, revealing gene expression patterns. - Fluorescence In Situ Hybridization (FISH): Uses fluorescent probes to detect specific DNA or RNA sequences in tissues. - Western Blotting: Analyzes protein expression and post-translational modifications in tissue extracts. - Confocal Microscopy: Provides high-resolution images of signaling molecules in tissues using fluorescently labeled antibodies or probes.
