The procedure for conducting a migration assay generally involves the following steps:
1. Preparation of Cells: Cells are cultured and maintained under appropriate conditions until they reach the required confluency. 2. Setting Up the Assay: Depending on the type of assay, cells are either seeded into a chamber, a scratch is made, or they are introduced into microfluidic devices. 3. Inducing Migration: Cells are exposed to stimuli such as chemoattractants or mechanical injury. 4. Observation and Analysis: The movement of cells is monitored using microscopy and quantified using image analysis software.
