What are the Key Types of In Vitro Migration Assays?
There are several types of in vitro migration assays, each with unique applications:
1. Boyden Chamber Assay: This is a widely used technique where cells migrate through a porous membrane towards a chemoattractant. It is often employed to study chemotaxis.
2. Scratch Wound Assay: Also known as the wound healing assay, this method involves creating a "scratch" in a cell monolayer and observing the migration of cells to close the wound.
3. Microfluidic Devices: These advanced systems use microchannels to mimic the cellular environment more accurately. They can simulate complex gradients and flow conditions.
