Several factors can influence RNA integrity in histological samples:
1. Tissue Handling: Improper handling can lead to RNA degradation by RNases. 2. Fixation: The choice of fixative (e.g., formalin) and fixation time can impact RNA quality. 3. Storage: Conditions such as temperature and duration of storage can affect RNA stability. 4. Processing: Techniques like paraffin embedding and sectioning can introduce artifacts that compromise RNA integrity.
