Lipid mediators are studied in histology through advanced techniques such as immunohistochemistry, in situ hybridization, and mass spectrometry imaging. Immunohistochemistry can be used to detect the presence of enzymes involved in lipid mediator synthesis, such as COX-2 and 5-LOX, as well as the lipid mediators themselves. Mass spectrometry imaging allows for the visualization and quantification of lipid mediators within tissue sections, providing insights into their spatial distribution and concentration.
