To achieve optimal results, several best practices should be followed:
Choose the appropriate decalcifying agent based on the tissue type and the urgency of the analysis. Monitor the decalcification process closely to prevent over or under-decalcification. Perform periodic checks using physical, chemical, or radiographic methods to determine the end-point. Maintain proper pH levels, especially when using acids. Post-decalcification, thoroughly wash the tissue to remove any residual decalcifying agent.
