In histology, gene fusions can be detected using various techniques:
- Fluorescence In Situ Hybridization (FISH): This technique uses fluorescent probes that bind to specific gene sequences, allowing visualization under a fluorescence microscope. - Immunohistochemistry (IHC): Antibodies specific to the proteins produced by fused genes can be used to stain tissue sections, helping to identify the presence of these proteins. - Polymerase Chain Reaction (PCR): This molecular technique amplifies DNA sequences, making it easier to detect gene fusions at a genetic level.
