Why are Freeze-Thaw Cycles Important in Histology?
Freeze-thaw cycles are crucial in histology for several reasons: - Sample Preservation: Freezing tissue samples can halt metabolic processes and enzymatic activities, effectively preserving the sample in its current state. - Cell Lysis: Repeated freeze-thaw can disrupt cell membranes, making it easier to extract intracellular components like proteins and nucleic acids. - Artifact Reduction: Proper freeze-thaw techniques can minimize the formation of ice crystals, which can cause artifacts in histological samples.
