freeze thaw cycles

How are Freeze-Thaw Cycles Performed?

The process typically involves the following steps:
1. Sample Preparation: Tissue samples are embedded in a suitable medium like OCT compound before freezing.
2. Freezing: The sample is rapidly frozen using liquid nitrogen, dry ice, or a specialized freezing machine.
3. Thawing: The sample is then allowed to thaw at room temperature or in a water bath.
4. Repetition: This cycle of freezing and thawing is repeated multiple times, usually 3-5 cycles, depending on the specific requirements.

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