The process typically involves the following steps: 1. Sample Preparation: Tissue samples are embedded in a suitable medium like OCT compound before freezing. 2. Freezing: The sample is rapidly frozen using liquid nitrogen, dry ice, or a specialized freezing machine. 3. Thawing: The sample is then allowed to thaw at room temperature or in a water bath. 4. Repetition: This cycle of freezing and thawing is repeated multiple times, usually 3-5 cycles, depending on the specific requirements.