What Techniques are Used to Study TGF-β in Histology?
Studying TGF-β in histology involves various techniques such as immunohistochemistry (IHC) to detect TGF-β protein levels in tissue samples. In situ hybridization can be used to localize TGF-β mRNA within tissues. Additionally, Western blotting and ELISA are employed to quantify TGF-β levels in tissue extracts. Advanced microscopy techniques, including confocal and electron microscopy, provide detailed visualization of TGF-β distribution and its effects on cellular and tissue architecture.
