Several clearing agents are commonly used in histology:
1. BABB (Benzyl Alcohol and Benzyl Benzoate): Effective for clearing large samples but can cause tissue shrinkage. 2. CLARITY: Uses hydrogel embedding and electrophoretic tissue clearing, ideal for preserving protein and nucleic acid integrity. 3. Scale: A hyperhydrating agent that is gentle on tissues, suitable for long-term imaging. 4. SeeDB: An aqueous-based agent that is easy to use and preserves fluorescent signals well.
